› 2wsm

X-ray diffraction
2.3Å resolution

Crystal structure of Hydrogenase Maturation Factor HypB From Archaeoglobus Fulgidus

Released:
DOI: 10.2210/pdb2wsm/pdb
PDB entry 2wsm coloured by chain, front view.
PDB entry 2wsm coloured by chain, side view.
PDB entry 2wsm coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Structural basis for GTP-dependent dimerization of hydrogenase maturation factor HypB.
Chan KH, Li T, Wong CO, Wong KB
PLoS One 7 e30547 (2012)
PMID: 22276211

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CobW/HypB/UreG nucleotide-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 24.7 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28903 (Residues: 1-221; Coverage: 100%)
Gene name: AF_1368
Sequence domains: CobW/HypB/UreG, nucleotide-binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21212
Unit cell:
a: 72.494Å b: 82.325Å c: 68.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Specific metal recognition in nickel trafficking.
Higgins et al. (2012)
1 more

2 mentions without citation

Structural basis for GTP-dependent dimerization of hydrogenase maturation factor HypB.
Chan et al. (2012)
1 more