› 2wt9

X-ray diffraction
1.65Å resolution

Acinetobacter baumanii nicotinamidase pyrazinamidease

Released:
DOI: 10.2210/pdb2wt9/pdb
PDB entry 2wt9 coloured by chain, front view.
PDB entry 2wt9 coloured by chain, side view.
PDB entry 2wt9 coloured by chain, top view.
Source organism: Acinetobacter baumannii AYE
Primary publication:
Specificity and mechanism of Acinetobacter baumanii nicotinamidase: implications for activation of the front-line tuberculosis drug pyrazinamide.
Fyfe PK, Rao VA, Zemla A, Cameron S, Hunter WN
Angew Chem Int Ed Engl 48 9176-9 (2009)
PMID: 19859929

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NICOTINAMIDASE Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26 KDa
Source organism: Acinetobacter baumannii AYE
Expression system: Escherichia coli BL21(DE3)
Structure domains: Isochorismatase-like

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 5 x GOL
Ligand NIO 2 x NIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 46.64Å b: 46.89Å c: 59.48Å
α: 113.19° β: 88.25° γ: 115.61°
R-values:
R R work R free
0.182 0.179 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Amide Bond Activation of Biological Molecules.
Mahesh et al. (2018)
1 more

9 mentions without citation

Crystal structure of the pyrazinamidase of Mycobacterium tuberculosis: insights into natural and acquired resistance to pyrazinamide.
Petrella et al. (2011)
8 more