› 2wta

X-ray diffraction
1.7Å resolution

Acinetobacter baumanii nicotinamidase pyrazinamidease

Released:
DOI: 10.2210/pdb2wta/pdb
PDB entry 2wta coloured by chain, front view.
PDB entry 2wta coloured by chain, side view.
PDB entry 2wta coloured by chain, top view.
Source organism: Acinetobacter baumannii AYE
Primary publication:
Specificity and mechanism of Acinetobacter baumanii nicotinamidase: implications for activation of the front-line tuberculosis drug pyrazinamide.
Fyfe PK, Rao VA, Zemla A, Cameron S, Hunter WN
Angew Chem Int Ed Engl 48 9176-9 (2009)
PMID: 19859929

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NICOTINAMIDASE Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26 KDa
Source organism: Acinetobacter baumannii AYE
Expression system: Escherichia coli BL21(DE3)
Structure domains: Isochorismatase-like

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand VGL 1 x VGL
Ligand GOL 3 x GOL
Ligand CL 3 x CL
1 modified residue:
Ligand CAF 1 x CAF

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Unit cell:
a: 50.688Å b: 46.507Å c: 107.602Å
α: 90° β: 101.97° γ: 90°
R-values:
R R work R free
0.149 0.147 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Amide Bond Activation of Biological Molecules.
Mahesh et al. (2018)
1 more

5 mentions without citation

The Riemerella anatipestifer AS87_01735 Gene Encodes Nicotinamidase PncA, an Important Virulence Factor.
Wang et al. (2016)
4 more