› 2wte

X-ray diffraction
1.8Å resolution

The structure of the CRISPR-associated protein, Csa3, from Sulfolobus solfataricus at 1.8 angstrom resolution.

Released:
DOI: 10.2210/pdb2wte/pdb
PDB entry 2wte coloured by chain, front view.
PDB entry 2wte coloured by chain, side view.
PDB entry 2wte coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
The structure of the CRISPR-associated protein Csa3 provides insight into the regulation of the CRISPR/Cas system.
Lintner NG, Frankel KA, Tsutakawa SE, Alsbury DL, Copié V, Young MJ, Tainer JA, Lawrence CM
J Mol Biol 405 939-55 (2011)
PMID: 21093452

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR locus-related putative DNA-binding protein Csa3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 28.23 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97Y88 (Residues: 1-237; Coverage: 100%)
Gene names: SSO1445, csa3
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 53.426Å b: 89.853Å c: 102.577Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation.
Bhaya et al. (2011)
11 more

7 mentions without citation

Evolution and classification of the CRISPR-Cas systems.
Makarova et al. (2011)
6 more