› 2wtn

X-ray diffraction
2.1Å resolution

Ferulic Acid bound to Est1E from Butyrivibrio proteoclasticus

Released:
DOI: 10.2210/pdb2wtn/pdb
PDB entry 2wtn coloured by chain, front view.
PDB entry 2wtn coloured by chain, side view.
PDB entry 2wtn coloured by chain, top view.
Source organism: Butyrivibrio proteoclasticus
Primary publication:
Structural and functional characterization of a promiscuous feruloyl esterase (Est1E) from the rumen bacterium Butyrivibrio proteoclasticus.
Goldstone DC, Villas-Bôas SG, Till M, Kelly WJ, Attwood GT, Arcus VL
Proteins 78 1457-69 (2010)
PMID: 20058325

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Est1e Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 28.06 KDa
Source organism: Butyrivibrio proteoclasticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D2YW37 (Residues: 1-251; Coverage: 100%)
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand GOL 6 x GOL
Ligand FER 2 x FER
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU-300R
Spacegroup: C2
Unit cell:
a: 144.279Å b: 48.177Å c: 97.035Å
α: 90° β: 127.74° γ: 90°
R-values:
R R work R free
0.167 0.164 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The release and catabolism of ferulic acid in plant cell wall by rumen microbes: A review.
Wang et al. (2022)
31 other articles

5 mentions without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)
4 more