› 2wts

X-ray diffraction
1.3Å resolution

Crystal structure of sortase C-1 (SrtC-1) mutant H131D from S. pneumoniae

Released:
DOI: 10.2210/pdb2wts/pdb
PDB entry 2wts coloured by chain, front view.
PDB entry 2wts coloured by chain, side view.
PDB entry 2wts coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Sortase activity is controlled by a flexible lid in the pilus biogenesis mechanism of gram-positive pathogens.
Manzano C, Izoré T, Job V, Di Guilmi AM, Dessen A
Biochemistry 48 10549-57 (2009)
PMID: 19810750

Function and Biology Details

Reaction catalysed: 
Peptidyl-tRNA(1) + aminoacyl-tRNA(2) = tRNA(1) + peptidyl(aminoacyl-tRNA(2))
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative sortase Chains: A, B
Molecule details ›
Chains: A, B
Length: 213 amino acids
Theoretical weight: 23.79 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2UNQ9 (Residues: 17-228; Coverage: 76%)
Gene name: SP_0466
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand ALA 1 x ALA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 68.348Å b: 70.523Å c: 87.278Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.195
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Sortase enzymes in Gram-positive bacteria.
Spirig et al. (2011)
9 more

2 mentions without citation

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
1 more