2wtu

X-ray diffraction
3.4Å resolution

Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-115916 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 800 amino acids
Theoretical weight: 89.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli B
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
Structure domains:
DNA Chain: E
Molecule details ›
Chain: E
Length: 16 nucleotides
Theoretical weight: 4.88 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA Chain: F
Molecule details ›
Chain: F
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 91.105Å b: 137.89Å c: 161.446Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.263
Expression systems:
  • Escherichia coli B
  • Not provided