› 2wu9

X-ray diffraction
1.5Å resolution

Crystal structure of peroxisomal KAT2 from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb2wu9/pdb
PDB entry 2wu9 coloured by chain, front view.
PDB entry 2wu9 coloured by chain, side view.
PDB entry 2wu9 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Peroxisomal plant 3-ketoacyl-CoA thiolase structure and activity are regulated by a sensitive redox switch.
Pye VE, Christensen CE, Dyer JH, Arent S, Henriksen A
J Biol Chem 285 24078-88 (2010)
PMID: 20463027

Function and Biology Details

Reaction catalysed: 
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176396 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-ketoacyl-CoA thiolase 2, peroxisomal Chains: A, B
Molecule details ›
Chains: A, B
Length: 442 amino acids
Theoretical weight: 46.6 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56WD9 (Residues: 36-462; Coverage: 92%)
Gene names: At2g33150, F25I18.11, KAT2, PED1
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P21
Unit cell:
a: 60.831Å b: 86.67Å c: 72.75Å
α: 90° β: 106.71° γ: 90°
R-values:
R R work R free
0.151 0.15 0.178
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The peroxisome: an update on mysteries.
Islinger et al. (2012)
3 more

5 mentions without citation

Peroxisomal plant 3-ketoacyl-CoA thiolase structure and activity are regulated by a sensitive redox switch.
Pye et al. (2010)
4 more