› 2wv1

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE HLYIIR MUTANT PROTEIN WITH RESIDUES 169-186 SUBSTITUTED BY A LINKER CONTAINING TWO THROMBIN SITES

Released:
DOI: 10.2210/pdb2wv1/pdb
PDB entry 2wv1 coloured by chain, front view.
PDB entry 2wv1 coloured by chain, side view.
PDB entry 2wv1 coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Structural investigation of transcriptional regulator HlyIIR: influence of a disordered region on protein fold and dimerization.
Kovalevskiy OV, Solonin AS, Antson AA
Proteins 78 1870-7 (2010)
PMID: 20225260

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 23.05 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7X506 (Residues: 4-168, 170-170, 187-201; Coverage: 90%)
Gene names: CQZ91_02635, hlyIIR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 52.315Å b: 70.473Å c: 139.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.236
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Proteopedia: a status report on the collaborative, 3D web-encyclopedia of proteins and other biomolecules.
Prilusky et al. (2011)

1 mention without citation

Streptococcus pneumoniae Sialidase SpNanB-Catalyzed One-Pot Multienzyme (OPME) Synthesis of 2,7-Anhydro-Sialic Acids as Selective Sialidase Inhibitors.
Xiao et al. (2018)