› 2wv9

X-ray diffraction
2.75Å resolution

Crystal Structure of the NS3 protease-helicase from Murray Valley encephalitis virus

Released:
DOI: 10.2210/pdb2wv9/pdb
PDB entry 2wv9 coloured by chain, front view.
PDB entry 2wv9 coloured by chain, side view.
PDB entry 2wv9 coloured by chain, top view.
Source organism: Murray valley encephalitis virus (strain MVE-1-51)
Primary publication:
Crystal structure of a novel conformational state of the flavivirus NS3 protein: implications for polyprotein processing and viral replication.
Assenberg R, Mastrangelo E, Walter TS, Verma A, Milani M, Owens RJ, Stuart DI, Grimes JM, Mancini EJ
J Virol 83 12895-906 (2009)
PMID: 19793813

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease NS3 Chain: A
Molecule details ›
Chain: A
Length: 673 amino acids
Theoretical weight: 74.06 KDa
Source organism: Murray valley encephalitis virus (strain MVE-1-51)
Expression system: Escherichia coli
UniProt:
  • Canonical: P05769 (Residues: 1421-2122; Coverage: 20%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 41.91Å b: 105.46Å c: 80.068Å
α: 90° β: 97.42° γ: 90°
R-values:
R R work R free
0.268 0.267 0.3
Expression system: Escherichia coli

Quick links

Citations

21 review citations

The flavivirus NS2B-NS3 protease-helicase as a target for antiviral drug development.
Luo et al. (2015)
20 more

19 mentions without citation

Molecular Insights into the Flavivirus Replication Complex.
van den Elsen et al. (2021)
18 more