2wvm

X-ray diffraction
2.98Å resolution

H309A mutant of Mannosyl-3-phosphoglycerate synthase from Thermus thermophilus HB27 in complex with GDP-alpha-D-Mannose and Mg(II)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-180876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannosyl-3-phosphogylcerate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 43.58 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q72K30 (Residues: 1-391; Coverage: 100%)
Gene name: TT_C0588
Sequence domains: Mannosyl-3-phosphoglycerate synthase (osmo_MPGsynth)
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 112.766Å b: 112.766Å c: 199.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.232
Expression system: Escherichia coli BL21