› 2wvt

X-ray diffraction
1.8Å resolution

Crystal structure of an alpha-L-fucosidase GH29 from Bacteroides thetaiotaomicron in complex with a novel iminosugar fucosidase inhibitor

Released:
DOI: 10.2210/pdb2wvt/pdb
PDB entry 2wvt coloured by chain, front view.
PDB entry 2wvt coloured by chain, side view.
PDB entry 2wvt coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Analysis of the reaction coordinate of alpha-L-fucosidases: a combined structural and quantum mechanical approach.
Lammerts van Bueren A, Ardèvol A, Fayers-Kerr J, Luo B, Zhang Y, Sollogoub M, Blériot Y, Rovira C, Davies GJ
J Am Chem Soc 132 1804-6 (2010)
PMID: 20092273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-L-fucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 443 amino acids
Theoretical weight: 51.33 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A3I4 (Residues: 31-473; Coverage: 97%)
Gene name: BT_2970
Sequence domains: Alpha-L-fucosidase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FHN 2 x FHN
Ligand GOL 5 x GOL
Ligand IMD 4 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Unit cell:
a: 104.83Å b: 85.16Å c: 121.285Å
α: 90° β: 108.73° γ: 90°
R-values:
R R work R free
0.209 0.207 0.245
Expression system: Escherichia coli BL21

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Citations

5 review citations

Mucin glycan foraging in the human gut microbiome.
Tailford et al. (2015)
4 more

5 mentions without citation

Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
Romero Victorica et al. (2020)
4 more