› 2wwb

Electron Microscopy
6.48Å resolution

CRYO-EM STRUCTURE OF THE MAMMALIAN SEC61 COMPLEX BOUND TO THE ACTIVELY TRANSLATING WHEAT GERM 80S RIBOSOME

Released:
DOI: 10.2210/pdb2wwb/pdb
PDB entry 2wwb coloured by chain, front view.
PDB entry 2wwb coloured by chain, side view.
PDB entry 2wwb coloured by chain, top view.
Source organisms:
Primary publication:
Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome.
Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart J, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon EC, Beckmann R
Science 326 1369-73 (2009)
PMID: 19933108
Related structures: EMD-1652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-137857 (preferred)
Entry contents:
11 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (15 distinct):
Protein transport protein Sec61 subunit alpha isoform 1 Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 52.28 KDa
Source organism: Canis lupus familiaris
UniProt:
  • Canonical: P38377 (Residues: 1-476; Coverage: 100%)
Gene name: SEC61A1
Sequence domains:
Protein transport protein Sec61 subunit gamma Chain: B
Molecule details ›
Chain: B
Length: 68 amino acids
Theoretical weight: 7.75 KDa
Source organism: Canis lupus familiaris
UniProt:
  • Canonical: P60058 (Residues: 1-68; Coverage: 100%)
Gene name: SEC61G
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Protein transport protein Sec61 subunit beta Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 9.99 KDa
Source organism: Canis lupus familiaris
UniProt:
  • Canonical: P60467 (Residues: 1-96; Coverage: 100%)
Gene name: SEC61B
Sequence domains: Sec61beta family
Large ribosomal subunit protein uL4B Chain: H
Molecule details ›
Chain: H
Length: 362 amino acids
Theoretical weight: 39.13 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P49626 (Residues: 1-362; Coverage: 100%)
Gene names: RPL2B, RPL4B, YD8119.17, YDR012W
Sequence domains:
Large ribosomal subunit protein uL22A Chain: I
Molecule details ›
Chain: I
Length: 184 amino acids
Theoretical weight: 20.59 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P05740 (Residues: 1-184; Coverage: 100%)
Gene names: RPL17, RPL17A, RPL20A, YKL180W
Sequence domains: Ribosomal protein L22p/L17e
60S RIBOSOMAL PROTEIN L19 Chain: J
Molecule details ›
Chain: J
Length: 189 amino acids
Theoretical weight: 21.76 KDa
Source organism: Triticum aestivum
Large ribosomal subunit protein uL23 Chain: K
Molecule details ›
Chain: K
Length: 142 amino acids
Theoretical weight: 15.79 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P04456 (Residues: 1-142; Coverage: 100%)
Gene names: RPL25, YOL127W
Sequence domains:
Large ribosomal subunit protein uL24A Chain: L
Molecule details ›
Chain: L
Length: 127 amino acids
Theoretical weight: 14.27 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P05743 (Residues: 1-127; Coverage: 100%)
Gene names: L8300.4, RPL26, RPL26A, RPL33A, YLR344W
Sequence domains:
Large ribosomal subunit protein eL31A Chain: M
Molecule details ›
Chain: M
Length: 113 amino acids
Theoretical weight: 12.98 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P0C2H8 (Residues: 1-113; Coverage: 100%)
Gene names: D2478, RPL31A, RPL34, RPL34A, YDL075W
Sequence domains: Ribosomal protein L31e
Large ribosomal subunit protein uL29A Chain: N
Molecule details ›
Chain: N
Length: 120 amino acids
Theoretical weight: 13.94 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P0CX84 (Residues: 1-120; Coverage: 100%)
Gene names: D1249, RPL35A, SOS1, YDL191W
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein eL39 Chain: O
Molecule details ›
Chain: O
Length: 51 amino acids
Theoretical weight: 6.36 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P04650 (Residues: 1-51; Coverage: 100%)
Gene names: J0360, RPL39, RPL46, SPB2, YJL189W
Sequence domains: Ribosomal L39 protein
5.8S ribosomal RNA Chain: D
Molecule details ›
Chain: D
Length: 63 nucleotides
Theoretical weight: 20.3 KDa
Sequence domains: 5.8S ribosomal RNA
25S ribosomal RNA Chain: E
Molecule details ›
Chain: E
Length: 34 nucleotides
Theoretical weight: 11.09 KDa
25S ribosomal RNA Chain: F
Molecule details ›
Chain: F
Length: 25 nucleotides
Theoretical weight: 8.05 KDa
25S ribosomal RNA Chain: G
Molecule details ›
Chain: G
Length: 14 nucleotides
Theoretical weight: 4.48 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.48Å
Relevant EMDB volumes: EMD-1652

Quick links

Citations

43 review citations

Mechanisms of Sec61/SecY-mediated protein translocation across membranes.
Park et al. (2012)
42 more

9 mentions without citation

Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum.
Erdmann et al. (2011)
8 more