› 2wxu

X-ray diffraction
1.8Å resolution

Clostridium perfringens alpha-toxin strain NCTC8237 mutant T74I

Released:
DOI: 10.2210/pdb2wxu/pdb
PDB entry 2wxu coloured by chain, front view.
PDB entry 2wxu coloured by chain, side view.
PDB entry 2wxu coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Comparison of a nontoxic variant of Clostridium perfringens α-toxin with the toxic wild-type strain.
Vachieri SG, Clark GC, Alape-Girón A, Flores-Díaz M, Justin N, Naylor CE, Titball RW, Basak AK
Acta Crystallogr D Biol Crystallogr 66 1067-74 (2010)
PMID: 20944240

Function and Biology Details

Reaction catalysed: 
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase C Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 42.6 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0TV31 (Residues: 29-398; Coverage: 100%)
Gene names: CPF_0042, cpa, plc
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CD 7 x CD
Ligand ZN 1 x ZN
Ligand CA 5 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 59.861Å b: 173.643Å c: 78.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.216
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cadmium-zinc exchange and their binary relationship in the structure of Zn-related proteins: a mini review.
Tang et al. (2014)

1 mention without citation

Local and global analysis of macromolecular atomic displacement parameters.
Masmaliyeva et al. (2020)