› 2wy2

Solution NMR

NMR structure of the IIAchitobiose-IIBchitobiose phosphoryl transition state complex of the N,N'-diacetylchitoboise brance of the E. coli phosphotransferase system.

Released:
DOI: 10.2210/pdb2wy2/pdb
PDB entry 2wy2 coloured by chain, ensemble of 64 models, front view.
PDB entry 2wy2 coloured by chain, ensemble of 64 models, side view.
PDB entry 2wy2 coloured by chain, ensemble of 64 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure of the IIAChitobiose-IIBChitobiose complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.
Jung YS, Cai M, Clore GM
J Biol Chem 285 4173-4184 (2010)
PMID: 19959833

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + N,N'-diacetylchitobiose(Side 1) = [protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159663 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system N,N'-diacetylchitobiose-specific EIIA component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 103 amino acids
Theoretical weight: 11.26 KDa
Source organism: Escherichia coli
Expression system: Milvago chimango chimango
UniProt:
  • Canonical: P69791 (Residues: 14-116; Coverage: 89%)
Gene names: JW1725, b1736, celC, chbA
Sequence domains: PTS system, Lactose/Cellobiose specific IIA subunit
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
PTS system N,N'-diacetylchitobiose-specific EIIB component Chain: D
Molecule details ›
Chain: D
Length: 103 amino acids
Theoretical weight: 11.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69795 (Residues: 3-105; Coverage: 97%)
Gene names: JW1727, b1738, celA, chbB
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PO3 1 x PO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression systems:
  • Milvago chimango chimango
  • Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure, dynamics and biophysics of the cytoplasmic protein-protein complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system.
Clore et al. (2013)
1 more

1 mention without citation

Solution structure of the IIAChitobiose-IIBChitobiose complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.
Jung et al. (2010)