› 2wz7

X-ray diffraction
2.48Å resolution

Crystal structure of the N-terminal domain of E.coli YbgF

Released:
DOI: 10.2210/pdb2wz7/pdb
PDB entry 2wz7 coloured by chain, front view.
PDB entry 2wz7 coloured by chain, side view.
PDB entry 2wz7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
TolA modulates the oligomeric status of YbgF in the bacterial periplasm.
Krachler AM, Sharma A, Cauldwell A, Papadakos G, Kleanthous C
J Mol Biol 403 270-85 (2010)
PMID: 20816983

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division coordinator CpoB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 75 amino acids
Theoretical weight: 8.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45955 (Residues: 35-109; Coverage: 32%)
Gene names: JW0732, b0742, cpoB, ybgF
Sequence domains: TolA binding protein trimerisation
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

6 bound ligands:
Ligand GOL 2 x GOL
Ligand ACT 2 x ACT
Ligand AUC 1 x AUC
Ligand NA 1 x NA
Ligand EDO 1 x EDO
Ligand AU 2 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 39.737Å b: 41.382Å c: 237.697Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.213 0.302
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The physiology of bacterial cell division.
Egan et al. (2013)
9 more

1 mention without citation

ARCIMBOLDO on coiled coils.
Caballero et al. (2018)