› 2wzi

X-ray diffraction
1.9Å resolution

BtGH84 D243N in complex with 5F-oxazoline

Released:
DOI: 10.2210/pdb2wzi/pdb
PDB entry 2wzi coloured by chain, front view.
PDB entry 2wzi coloured by chain, side view.
PDB entry 2wzi coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Visualizing the reaction coordinate of an O-GlcNAc hydrolase.
He Y, Macauley MS, Stubbs KA, Vocadlo DJ, Davies GJ
J Am Chem Soc 132 1807-9 (2010)
PMID: 20067256

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-GlcNAcase BT_4395 Chains: A, B
Molecule details ›
Chains: A, B
Length: 737 amino acids
Theoretical weight: 84.59 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q89ZI2 (Residues: 1-737; Coverage: 100%)
Gene name: BT_4395
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GOL 5 x GOL
Ligand CA 2 x CA
Ligand 5FN 2 x 5FN
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P1
Unit cell:
a: 51.38Å b: 93.82Å c: 98.88Å
α: 104.14° β: 94.06° γ: 103.08°
R-values:
R R work R free
0.186 0.184 0.223
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

O-GlcNAc signalling: implications for cancer cell biology.
Slawson et al. (2011)
12 more

3 mentions without citation

Recent and recurrent selective sweeps of the antiviral RNAi gene Argonaute-2 in three species of Drosophila.
Obbard et al. (2011)
2 more