› 2wzw

X-ray diffraction
1.8Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitroreductase NfnB Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 25.94 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0R6D0 (Residues: 1-234; Coverage: 100%)
Gene names: MSMEG_6505, MSMEI_6333, nfnB
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments

3 bound ligands:
Ligand FMN 2 x FMN
Ligand NDP 1 x NDP
Ligand PO4 3 x PO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I212121
Unit cell:
a: 85.55Å b: 115.61Å c: 119.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.172
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Molecular basis and mechanisms of drug resistance in Mycobacterium tuberculosis: classical and new drugs.
Almeida Da Silva et al. (2011)
12 more

5 mentions without citation

Crystal structure of reduced MsAcg, a putative nitroreductase from Mycobacterium smegmatis and a close homologue of Mycobacterium tuberculosis Acg.
Chauviac et al. (2012)
4 more