› 2x0e

X-ray diffraction
2.81Å resolution

Complex structure of WsaF with dTDP

Released:
DOI: 10.2210/pdb2x0e/pdb
PDB entry 2x0e coloured by chain, front view.
PDB entry 2x0e coloured by chain, side view.
PDB entry 2x0e coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structural basis of substrate binding in WsaF, a rhamnosyltransferase from Geobacillus stearothermophilus.
Steiner K, Hagelueken G, Messner P, Schäffer C, Naismith JH
J Mol Biol 397 436-47 (2010)
PMID: 20097205

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WsaF N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 413 amino acids
Theoretical weight: 48.23 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BG50 (Residues: 1-413; Coverage: 100%)
Gene name: wsaF
Sequence domains: WsaF, N-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TYD 2 x TYD
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 75.82Å b: 75.56Å c: 77.71Å
α: 90° β: 103.05° γ: 90°
R-values:
R R work R free
0.188 0.185 0.245
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Synthesis of lipopolysaccharide O-antigens by ABC transporter-dependent pathways.
Greenfield et al. (2012)
6 more

1 mention without citation

Structural basis of substrate binding in WsaF, a rhamnosyltransferase from Geobacillus stearothermophilus.
Steiner et al. (2010)