› 2x0f

X-ray diffraction
2.55Å resolution

Structure of WsaF in complex with dTDP-beta-L-Rha

Released:
DOI: 10.2210/pdb2x0f/pdb
PDB entry 2x0f coloured by chain, front view.
PDB entry 2x0f coloured by chain, side view.
PDB entry 2x0f coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structural basis of substrate binding in WsaF, a rhamnosyltransferase from Geobacillus stearothermophilus.
Steiner K, Hagelueken G, Messner P, Schäffer C, Naismith JH
J Mol Biol 397 436-47 (2010)
PMID: 20097205

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WsaF N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 413 amino acids
Theoretical weight: 48.23 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BG50 (Residues: 1-413; Coverage: 100%)
Gene name: wsaF
Sequence domains: WsaF, N-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand TRH 2 x TRH
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 75.94Å b: 75.65Å c: 77.48Å
α: 90° β: 102.78° γ: 90°
R-values:
R R work R free
0.188 0.186 0.23
Expression system: Escherichia coli

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Citations

7 review citations

Synthesis of lipopolysaccharide O-antigens by ABC transporter-dependent pathways.
Greenfield et al. (2012)
6 more

2 mentions without citation

The s-layer glycome-adding to the sugar coat of bacteria.
Ristl et al. (2011)
1 more