› 2x1a

X-ray diffraction
2.05Å resolution

Structure of Rna15 RRM with RNA bound (G)

Released:
DOI: 10.2210/pdb2x1a/pdb
PDB entry 2x1a coloured by chain, front view.
PDB entry 2x1a coloured by chain, side view.
PDB entry 2x1a coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the Rna15 RRM-RNA complex reveals the molecular basis of GU specificity in transcriptional 3'-end processing factors.
Pancevac C, Goldstone DC, Ramos A, Taylor IA
Nucleic Acids Res 38 3119-32 (2010)
PMID: 20097654

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150418 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
mRNA 3'-end-processing protein RNA15 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25299 (Residues: 16-111; Coverage: 32%)
Gene names: RNA15, YGL044C
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-R(*GP*UP*UP*GP*UP)-3' Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.56 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 37.037Å b: 74.967Å c: 31.508Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Structural insight into RNA recognition motifs: versatile molecular Lego building blocks for biological systems.
Muto et al. (2012)
5 more

9 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
8 more