› 2x1l

X-ray diffraction
2.3Å resolution

Crystal structure of Mycobacterium smegmatis methionyl-tRNA synthetase in complex with methionine and adenosine

Released:
DOI: 10.2210/pdb2x1l/pdb
PDB entry 2x1l coloured by chain, front view.
PDB entry 2x1l coloured by chain, side view.
PDB entry 2x1l coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Flexibility and communication within the structure of the Mycobacterium smegmatis methionyl-tRNA synthetase.
Ingvarsson H, Unge T
FEBS J 277 3947-62 (2010)
PMID: 20796028

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 524 amino acids
Theoretical weight: 59.18 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0R3E2 (Residues: 2-515; Coverage: 100%)
Gene names: MSMEG_5441, metG
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MET 3 x MET
Ligand ADN 3 x ADN
Ligand CXS 3 x CXS
Ligand 2HP 3 x 2HP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: C2
Unit cell:
a: 155.95Å b: 138.91Å c: 123.31Å
α: 90° β: 124.84° γ: 90°
R-values:
R R work R free
0.219 0.218 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Emergence and evolution.
Bullwinkle et al. (2014)
3 other articles

4 mentions without citation

Brucella melitensis Methionyl-tRNA-Synthetase (MetRS), a Potential Drug Target for Brucellosis.
Ojo et al. (2016)
3 more