› 2x1m

X-ray diffraction
2.8Å resolution

Crystal structure of Mycobacterium smegmatis methionyl-tRNA synthetase in complex with methionine

Released:
DOI: 10.2210/pdb2x1m/pdb
PDB entry 2x1m coloured by chain, front view.
PDB entry 2x1m coloured by chain, side view.
PDB entry 2x1m coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Flexibility and communication within the structure of the Mycobacterium smegmatis methionyl-tRNA synthetase.
Ingvarsson H, Unge T
FEBS J 277 3947-62 (2010)
PMID: 20796028

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 524 amino acids
Theoretical weight: 59.18 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0R3E2 (Residues: 2-515; Coverage: 100%)
Gene names: MSMEG_5441, metG
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MET 1 x MET
Ligand 2HP 1 x 2HP
Ligand CXS 1 x CXS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: R32
Unit cell:
a: 209.99Å b: 209.99Å c: 73.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Emergence and evolution.
Bullwinkle et al. (2014)
4 other articles

1 mention without citation

The crystal structure of the drug target Mycobacterium tuberculosis methionyl-tRNA synthetase in complex with a catalytic intermediate.
Barros-Álvarez et al. (2018)