› 2x1q

X-ray diffraction
1.06Å resolution

Gelsolin Nanobody

Released:
DOI: 10.2210/pdb2x1q/pdb
Model geometry
Fit model/data
PDB entry 2x1q coloured by chain, front view.
PDB entry 2x1q coloured by chain, side view.
PDB entry 2x1q coloured by chain, top view.
Source organism: Lama glama
Primary publication:
A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction.
Van den Abbeele A, De Clercq S, De Ganck A, De Corte V, Van Loo B, Soror SH, Srinivasan V, Steyaert J, Vandekerckhove J, Gettemans J
Cell Mol Life Sci 67 1519-35 (2010)
PMID: 20140750

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GELSOLIN NANOBODY Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.91 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 35.908Å b: 63.425Å c: 50.01Å
α: 90° β: 90.36° γ: 90°
R-values:
R R work R free
0.214 0.213 0.229
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein-Protein Interaction Analysis, and Protein Function Exploration.
Beghein et al. (2017)
8 more

7 mentions without citation

Structural basis for GABAA receptor potentiation by neurosteroids.
Miller et al. (2017)
6 more