› 2x2q

X-ray diffraction
1.9Å resolution

Crystal structure of an 'all locked' LNA duplex at 1.9 angstrom resolution

Released:
DOI: 10.2210/pdb2x2q/pdb
PDB entry 2x2q coloured by chain, front view.
PDB entry 2x2q coloured by chain, side view.
PDB entry 2x2q coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
The crystal structure of an 'All Locked' nucleic acid duplex.
Eichert A, Behling K, Betzel C, Erdmann VA, Fürste JP, Förster C
Nucleic Acids Res 38 6729-36 (2010)
PMID: 20530536

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196600 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
LOCKED NUCLEIC ACID DERIVED FROM TRNA SER ACCEPTOR STEM MICROHELIX Chains: A, C
Molecule details ›
Chains: A, C
Length: 7 nucleotides
Theoretical weight: 2.28 KDa
Source organism: synthetic construct
Expression system: Not provided
LOCKED NUCLEIC ACID DERIVED FROM TRNA SER ACCEPTOR STEM MICROHELIX Chains: B, D
Molecule details ›
Chains: B, D
Length: 7 nucleotides
Theoretical weight: 2.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand NCO 1 x NCO
Ligand MG 1 x MG
Ligand CAC 1 x CAC
3 modified residues:
Ligand TLN 4 x TLN
Ligand LCA 4 x LCA
Ligand LCG 10 x LCG

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 77.913Å b: 40.737Å c: 30.059Å
α: 90° β: 91.02° γ: 90°
R-values:
R R work R free
0.226 0.224 0.264
Expression system: Not provided

Quick links

Citations

3 review citations

Locked vs. unlocked nucleic acids (LNA vs. UNA): contrasting structures work towards common therapeutic goals.
Campbell et al. (2011)
2 more

6 mentions without citation

Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective.
Zhu et al. (2021)
5 more