› 2x47

X-ray diffraction
1.7Å resolution

Crystal structure of human MACROD1

Released:
DOI: 10.2210/pdb2x47/pdb
PDB entry 2x47 coloured by chain, front view.
PDB entry 2x47 coloured by chain, side view.
PDB entry 2x47 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of macrodomain proteins as novel O-acetyl-ADP-ribose deacetylases.
Chen D, Vollmar M, Rossi MN, Phillips C, Kraehenbuehl R, Slade D, Mehrotra PV, von Delft F, Crosthwaite SK, Gileadi O, Denu JM, Ahel I
J Biol Chem 286 13261-71 (2011)
PMID: 21257746

Function and Biology Details

Reaction catalysed: 
2''-O-acetyl-ADP-D-ribose + H(2)O = ADP-D-ribose + acetate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribose glycohydrolase MACROD1 Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BQ69 (Residues: 91-325; Coverage: 72%)
Gene names: LRP16, MACROD1
Sequence domains: Macro domain
Structure domains: Leucine Aminopeptidase, subunit E, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 45.92Å b: 63.41Å c: 75.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs.
Gibson et al. (2012)
34 more

14 mentions without citation

A family of macrodomain proteins reverses cellular mono-ADP-ribosylation.
Jankevicius et al. (2013)
13 more