› 2x4a

X-ray diffraction
2.54Å resolution

Crystal structure of the C-terminal domain of InvA

Released:
DOI: 10.2210/pdb2x4a/pdb
PDB entry 2x4a coloured by chain, front view.
PDB entry 2x4a coloured by chain, side view.
PDB entry 2x4a coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Crystal structure of the C-terminal domain of the Salmonella type III secretion system export apparatus protein InvA.
Worrall LJ, Vuckovic M, Strynadka NC
Protein Sci 19 1091-6 (2010)
PMID: 20306492

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Invasion protein InvA Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 38.15 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A1I3 (Residues: 357-685; Coverage: 48%)
Gene names: STM2896, invA
Sequence domains: FHIPEP family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CXS 1 x CXS
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3221
Unit cell:
a: 79.17Å b: 79.17Å c: 127.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
15 more

2 mentions without citation

Architecture of the major component of the type III secretion system export apparatus.
Abrusci et al. (2013)
1 more