› 2x52

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ ISOLECTIN 3 IN COMPLEX WITH A SYNTHETIC DIVALENT CARBOHYDRATE LIGAND

Released:
DOI: 10.2210/pdb2x52/pdb
PDB entry 2x52 coloured by chain, front view.
PDB entry 2x52 coloured by chain, side view.
PDB entry 2x52 coloured by chain, top view.
Source organism: Triticum aestivum
Primary publication:
Structural basis of multivalent binding to wheat germ agglutinin.
Schwefel D, Maierhofer C, Beck JG, Seeberger S, Diederichs K, Möller HM, Welte W, Wittmann V
J Am Chem Soc 132 8704-19 (2010)
PMID: 20527753

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Agglutinin isolectin 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 17.22 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P10969 (Residues: 1-171; Coverage: 92%)
Sequence domains: Chitin recognition protein
Structure domains: Endochitinase-like

Ligands and Environments

2 bound ligands:
Ligand GYT 4 x GYT
Ligand GOL 1 x GOL
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R3
Unit cell:
a: 101.33Å b: 101.33Å c: 144.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.158 0.171

Quick links

Citations

14 review citations

Bridging lectin binding sites by multivalent carbohydrates.
Wittmann et al. (2013)
13 more

7 mentions without citation

Glycosylated gold nanoparticles in point of care diagnostics: from aggregation to lateral flow.
Baker et al. (2022)
6 more