› 2x5j

X-ray diffraction
2.3Å resolution

Crystal structure of the Apoform of the D-Erythrose-4-phosphate dehydrogenase from E. coli

Released:
DOI: 10.2210/pdb2x5j/pdb
PDB entry 2x5j coloured by chain, front view.
PDB entry 2x5j coloured by chain, side view.
PDB entry 2x5j coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Moniot S, Didierjean C, Boschi-Muller S, Branlant G, Corbier C

Function and Biology Details

Reaction catalysed: 
D-erythrose 4-phosphate + NAD(+) + H(2)O = 4-phosphoerythronate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-erythrose-4-phosphate dehydrogenase Chains: O, P, Q, R
Molecule details ›
Chains: O, P, Q, R
Length: 339 amino acids
Theoretical weight: 37.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A9B6 (Residues: 1-339; Coverage: 100%)
Gene names: JW2894, b2927, epd, gapB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 86.3Å b: 110.7Å c: 138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.266
Expression system: Escherichia coli K-12

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