› 2x71

X-ray diffraction
2.1Å resolution

Structural basis for the interaction of lactivicins with serine beta- lactamases

Released:
DOI: 10.2210/pdb2x71/pdb
PDB entry 2x71 coloured by chain, front view.
PDB entry 2x71 coloured by chain, side view.
PDB entry 2x71 coloured by chain, top view.
Source organism: Bacillus licheniformis
Primary publication:
Structural basis for the interaction of lactivicins with serine beta-lactamases.
Brown T, Charlier P, Herman R, Schofield CJ, Sauvage E
J Med Chem 53 5890-4 (2010)
PMID: 20593835

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 29.51 KDa
Source organism: Bacillus licheniformis
UniProt:
  • Canonical: P94458 (Residues: 1-265; Coverage: 100%)
Gene name: bla
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand L4C 1 x L4C
Ligand EOH 4 x EOH
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 46.652Å b: 104.089Å c: 62.838Å
α: 90° β: 94.45° γ: 90°
R-values:
R R work R free
0.189 0.187 0.227

Quick links

Citations

8 review citations

Electrophilic natural products and their biological targets.
Gersch et al. (2012)
7 more

1 mention without citation

X-ray evidence of a native state with increased compactness populated by tryptophan-less B. licheniformis β-lactamase.
Risso et al. (2012)