› 2x75

X-ray diffraction
2.5Å resolution

Staphylococcus aureus adenylosuccinate lyase

Released:
DOI: 10.2210/pdb2x75/pdb
PDB entry 2x75 coloured by chain, front view.
PDB entry 2x75 coloured by chain, side view.
PDB entry 2x75 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structure of Staphylococcus aureus adenylosuccinate lyase (PurB) and assessment of its potential as a target for structure-based inhibitor discovery.
Fyfe PK, Dawson A, Hutchison MT, Cameron S, Hunter WN
Acta Crystallogr D Biol Crystallogr 66 881-8 (2010)
PMID: 20693687

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 49.67 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7A0G9 (Residues: 1-431; Coverage: 100%)
Gene names: MW1849, purB
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand OXL 1 x OXL
Ligand AMP 1 x AMP
Ligand P6G 1 x P6G
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I222
Unit cell:
a: 81.647Å b: 121.265Å c: 105.727Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.214 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

The genome and transcriptome of the zoonotic hookworm Ancylostoma ceylanicum identify infection-specific gene families.
Schwarz et al. (2015)
8 more

1 mention without citation

Structure of Staphylococcus aureus adenylosuccinate lyase (PurB) and assessment of its potential as a target for structure-based inhibitor discovery.
Fyfe et al. (2010)