› 2x77

X-ray diffraction
2.1Å resolution

Crystal Structure of Leishmania major ADP ribosylation factor-like 1.

Released:
DOI: 10.2210/pdb2x77/pdb
PDB entry 2x77 coloured by chain, front view.
PDB entry 2x77 coloured by chain, side view.
PDB entry 2x77 coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Crystal structure of Leishmania major ADP-ribosylation factor-like 1 and a classification of related GTPase family members in this Kinetoplastid.
Fleming JR, Dawson A, Hunter WN
Mol Biochem Parasitol 174 141-4 (2010)
PMID: 20801163

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor-like protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.01 KDa
Source organism: Leishmania major
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4QEJ1 (Residues: 1-187; Coverage: 100%)
Gene names: ARL-1, LMJF_17_0070
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 35.286Å b: 78.944Å c: 58.034Å
α: 90° β: 93.1° γ: 90°
R-values:
R R work R free
0.218 0.214 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Crystal structure of the C-terminal domain of tubulin-binding cofactor C from Leishmania major.
Barrack et al. (2015)

4 mentions without citation

Structure of Plasmodium falciparum ADP-ribosylation factor 1.
Cook et al. (2010)
3 more