Function and Biology Details
Reaction catalysed:
Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO(2)
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase
- Thiamin diphosphate-binding fold
- Thiamine pyrophosphate enzyme, TPP-binding
- Menaquinone biosynthesis protein MenD, middle domain
- DHS-like NAD/FAD-binding domain superfamily
- Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain
Structure analysis Details
Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Molecule details ›
Chains: A, B, C, D
Length: 604 amino acids
Theoretical weight: 66.95 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 604 amino acids
Theoretical weight: 66.95 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P23970 (Residues: 1-580; Coverage: 100%)
Sequence domains:
- Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
- Middle domain of thiamine pyrophosphate
- Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Ligands and Environments
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
ESRF BEAMLINE ID23-1
Spacegroup:
P212121
Expression system: Escherichia coli BL21(DE3)
