› 2x7m

X-ray diffraction
1.5Å resolution

Crystal structure of Archaemetzincin (amzA) from Methanopyrus kandleri at 1.5 A resolution

Released:
DOI: 10.2210/pdb2x7m/pdb
PDB entry 2x7m coloured by chain, front view.
PDB entry 2x7m coloured by chain, side view.
PDB entry 2x7m coloured by chain, top view.
Source organism: Methanopyrus kandleri AV19
Primary publication:
Crystal structure of archaemetzincin AmzA from Methanopyrus kandleri at 1.5 A resolution.
Waltersperger S, Widmer C, Wang M, Baumann U
Proteins 78 2720-3 (2010)
PMID: 20597090

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186311 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Archaemetzincin Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.03 KDa
Source organism: Methanopyrus kandleri AV19
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TXW1 (Residues: 1-175; Coverage: 100%)
Gene names: MK0548, amzA
Sequence domains: Peptidase family M54
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 50.51Å b: 55.08Å c: 58.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

New mini- zincin structures provide a minimal scaffold for members of this metallopeptidase superfamily.
Trame et al. (2014)
3 more

5 mentions without citation

Architecture and function of metallopeptidase catalytic domains.
Cerdà-Costa et al. (2014)
4 more