› 2x7x

X-ray diffraction
2.64Å resolution

Fructose binding periplasmic domain of hybrid two component system BT1754

Released:
DOI: 10.2210/pdb2x7x/pdb
PDB entry 2x7x coloured by chain, front view.
PDB entry 2x7x coloured by chain, side view.
PDB entry 2x7x coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Specificity of polysaccharide use in intestinal bacteroides species determines diet-induced microbiota alterations.
Sonnenburg ED, Zheng H, Joglekar P, Higginbottom SK, Firbank SJ, Bolam DN, Sonnenburg JL
Cell 141 1241-52 (2010)
PMID: 20603004

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183727 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 36.12 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8A6X1 (Residues: 29-343; Coverage: 34%)
Gene name: BT_1754
Sequence domains: Periplasmic binding protein domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 4 x PO4
Ligand FRU 2 x FRU
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P65
Unit cell:
a: 111.84Å b: 111.84Å c: 115.171Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

103 review citations

Interactions between the microbiota and the immune system.
Hooper et al. (2012)
102 more