› 2x7z

X-ray diffraction
2Å resolution

Crystal Structure of the SAP97 PDZ2 I342W C378A mutant protein domain

Released:
DOI: 10.2210/pdb2x7z/pdb
PDB entry 2x7z coloured by chain, front view.
PDB entry 2x7z coloured by chain, side view.
PDB entry 2x7z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The plastic energy landscape of protein folding: a triangular folding mechanism with an equilibrium intermediate for a small protein domain.
Haq SR, Jürgens MC, Chi CN, Koh CS, Elfström L, Selmer M, Gianni S, Jemth P
J Biol Chem 285 18051-9 (2010)
PMID: 20356847

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disks large homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 10.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12959 (Residues: 311-407; Coverage: 11%)
Gene name: DLG1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

2 bound ligands:
Ligand NH4 1 x NH4
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P4322
Unit cell:
a: 47.905Å b: 47.905Å c: 123.287Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Ligand binding by PDZ domains.
Chi et al. (2012)
3 more

8 mentions without citation

Nanopore Technology for the Application of Protein Detection.
Zeng et al. (2021)
7 more