› 2x8f

X-ray diffraction
1.9Å resolution

Native structure of Endo-1,5-alpha-L-arabinanases from Bacillus subtilis

Released:
DOI: 10.2210/pdb2x8f/pdb
PDB entry 2x8f coloured by chain, front view.
PDB entry 2x8f coloured by chain, side view.
PDB entry 2x8f coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases.
de Sanctis D, Inácio JM, Lindley PF, de Sá-Nogueira I, Bento I
FEBS J 277 4562-74 (2010)
PMID: 20883454

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154601 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular endo-alpha-(1->5)-L-arabinanase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 470 amino acids
Theoretical weight: 52.74 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42293 (Residues: 1-469; Coverage: 100%)
Gene names: BSU39330, J3A, abn2, yxiA
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand PO4 2 x PO4
Ligand CA 2 x CA
Ligand GOL 10 x GOL
Ligand NA 4 x NA
Ligand MPD 1 x MPD
Ligand TRS 1 x TRS
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 51.975Å b: 57.645Å c: 86.272Å
α: 82.31° β: 87.97° γ: 63.66°
R-values:
R R work R free
0.143 0.141 0.18
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Genomics review of holocellulose deconstruction by aspergilli.
Segato et al. (2014)
25 other articles

1 mention without citation

Dividing the Large Glycoside Hydrolase Family 43 into Subfamilies: a Motivation for Detailed Enzyme Characterization.
Mewis et al. (2016)