› 2x98

X-ray diffraction
1.7Å resolution

H.SALINARUM ALKALINE PHOSPHATASE

Released:
DOI: 10.2210/pdb2x98/pdb
PDB entry 2x98 coloured by chain, front view.
PDB entry 2x98 coloured by chain, side view.
PDB entry 2x98 coloured by chain, top view.
Source organism: Halobacterium salinarum R1
Primary publication:
Structural and biochemical characterization of a halophilic archaeal alkaline phosphatase.
Wende A, Johansson P, Vollrath R, Dyall-Smith M, Oesterhelt D, Grininger M
J Mol Biol 400 52-62 (2010)
PMID: 20438737

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-109195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 45.14 KDa
Source organism: Halobacterium salinarum R1
Expression system: Halobacterium salinarum R1
UniProt:
  • Canonical: B0R9W3 (Residues: 44-473; Coverage: 91%)
Gene names: OE_5192R, aph
Sequence domains: Alkaline phosphatase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand PO4 2 x PO4
Ligand ZN 4 x ZN
Ligand MG 11 x MG
Ligand NA 2 x NA
Ligand CL 13 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 72.143Å b: 152.524Å c: 86.799Å
α: 90° β: 110° γ: 90°
R-values:
R R work R free
0.163 0.16 0.198
Expression system: Halobacterium salinarum R1

Quick links

Citations

10 citation in other articles

Active site detection by spatial conformity and electrostatic analysis--unravelling a proteolytic function in shrimp alkaline phosphatase.
Chakraborty et al. (2011)
9 more

6 mentions without citation

Cellular function and molecular structure of ecto-nucleotidases.
Zimmermann et al. (2012)
5 more