› 2x9b

X-ray diffraction
2.92Å resolution

The filamentous phages fd and IF1 use different infection mechanisms

Released:
DOI: 10.2210/pdb2x9b/pdb
PDB entry 2x9b coloured by chain, front view.
PDB entry 2x9b coloured by chain, side view.
PDB entry 2x9b coloured by chain, top view.
Source organism: Escherichia phage If1
Primary publication:
The filamentous phages fd and IF1 use different mechanisms to infect Escherichia coli.
Lorenz SH, Jakob RP, Weininger U, Balbach J, Dobbek H, Schmid FX
J Mol Biol 405 989-1003 (2011)
PMID: 21110981

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Attachment protein G3P Chains: A, B
Molecule details ›
Chains: A, B
Length: 65 amino acids
Theoretical weight: 7.04 KDa
Source organism: Escherichia phage If1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O80297 (Residues: 17-81; Coverage: 15%)
Gene name: III
Sequence domains: Phage Coat Protein A
Structure domains: Phage FD Coat Protein,Membrane penetration domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6222
Unit cell:
a: 106.836Å b: 106.836Å c: 75.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.243 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Bacteriophage based probes for pathogen detection.
Singh et al. (2012)
4 more

1 mention without citation

Updates to the Integrated Protein-Protein Interaction Benchmarks: Docking Benchmark Version 5 and Affinity Benchmark Version 2.
Vreven et al. (2015)