› 2x9c

X-ray diffraction
2.45Å resolution

Crystal structure of a soluble PrgI mutant from Salmonella Typhimurium

Released:
DOI: 10.2210/pdb2x9c/pdb
PDB entry 2x9c coloured by chain, front view.
PDB entry 2x9c coloured by chain, side view.
PDB entry 2x9c coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Primary publication:
Protein refolding is required for assembly of the type three secretion needle.
Poyraz O, Schmidt H, Seidel K, Delissen F, Ader C, Tenenboim H, Goosmann C, Laube B, Thünemann AF, Zychlinsky A, Baldus M, Lange A, Griesinger C, Kolbe M
Nat Struct Mol Biol 17 788-92 (2010)
PMID: 20543831

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SPI-1 type 3 secretion system needle filament protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 83 amino acids
Theoretical weight: 9.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41784 (Residues: 1-80; Coverage: 100%)
Gene names: STM2873, prgI, sctF1
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3112
Unit cell:
a: 64.53Å b: 64.53Å c: 104.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
14 more

1 mention without citation

The blueprint of the type-3 injectisome.
Kosarewicz et al. (2012)