› 2x9k

X-ray diffraction
2.18Å resolution

Structure of a E.coli porin

Released:
DOI: 10.2210/pdb2x9k/pdb
PDB entry 2x9k coloured by chain, front view.
PDB entry 2x9k coloured by chain, side view.
PDB entry 2x9k coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Correlation between the OmpG secondary structure and its pH-dependent alterations monitored by FTIR.
Korkmaz-Ozkan F, Köster S, Kühlbrandt W, Mäntele W, Yildiz O
J Mol Biol 401 56-67 (2010)
PMID: 20561532

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160039 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin G Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 32.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76045 (Residues: 22-301; Coverage: 100%)
Gene names: JW1312, b1319, ompG
Sequence domains: Outer membrane protein G (OmpG)
Structure domains: monomeric porin ompg

Ligands and Environments

1 bound ligand:
Ligand BOG 9 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 55.61Å b: 70.96Å c: 119.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Strategies and perspectives in ion-channel engineering.
Grosse et al. (2011)
8 other articles

6 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
5 more