› 2xb5

X-ray diffraction
2.5Å resolution

Tet repressor (class D) in complex with 7-Iodotetracycline

Released:
DOI: 10.2210/pdb2xb5/pdb
PDB entry 2xb5 coloured by chain, front view.
PDB entry 2xb5 coloured by chain, side view.
PDB entry 2xb5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance.
Hinrichs W, Kisker C, Düvel M, Müller A, Tovar K, Hillen W, Saenger W
Science 264 418-20 (1994)
PMID: 8153629

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0ACT4 (Residues: 2-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand I7T 1 x I7T
Ligand MG 1 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: I4122
Unit cell:
a: 68.86Å b: 68.86Å c: 180.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.162 0.23
Expression system: Escherichia coli K-12

Quick links

Citations

40 review citations

Proton-dependent multidrug efflux systems.
Paulsen et al. (1996)
39 more

3 mentions without citation

An Effective Computational Method Incorporating Multiple Secondary Structure Predictions in Topology Determination for Cryo-EM Images.
Biswas et al. (2017)
2 more