› 2xba

X-ray diffraction
1.95Å resolution

Structure of Human Anaplastic Lymphoma Kinase in complex with PHA- E429

Released:
DOI: 10.2210/pdb2xba/pdb
PDB entry 2xba coloured by chain, front view.
PDB entry 2xba coloured by chain, side view.
PDB entry 2xba coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of anaplastic lymphoma kinase in complex with ATP competitive inhibitors.
Bossi RT, Saccardo MB, Ardini E, Menichincheri M, Rusconi L, Magnaghi P, Orsini P, Avanzi N, Borgia AL, Nesi M, Bandiera T, Fogliatto G, Bertrand JA
Biochemistry 49 6813-25 (2010)
PMID: 20695522

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ALK tyrosine kinase receptor Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 35.33 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UM73 (Residues: 1094-1407; Coverage: 20%)
Gene name: ALK
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 571 1 x 571
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 51.884Å b: 57.013Å c: 105.541Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.229
Expression system: Spodoptera frugiperda

Quick links

Citations

26 review citations

Mechanistic insight into ALK receptor tyrosine kinase in human cancer biology.
Hallberg et al. (2013)
25 more

5 mentions without citation

ALK and ROS1 as a joint target for the treatment of lung cancer: a review.
Puig de la Bellacasa et al. (2013)
4 more