› 2xbp

X-ray diffraction
1.2Å resolution

A novel signal transduction protein PII variant from Synechococcus elongatus PCC7942 indicates a two-step process for NAGK PII complex formation

Released:
DOI: 10.2210/pdb2xbp/pdb
Model geometry
Fit model/data
PDB entry 2xbp coloured by chain, front view.
PDB entry 2xbp coloured by chain, side view.
PDB entry 2xbp coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
A novel signal transduction protein P(II) variant from Synechococcus elongatus PCC 7942 indicates a two-step process for NAGK-P(II) complex formation.
Fokina O, Chellamuthu VR, Zeth K, Forchhammer K
J Mol Biol 399 410-21 (2010)
PMID: 20399792

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein P-II Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.5 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3F4 (Residues: 1-112; Coverage: 100%)
Gene names: Synpcc7942_0321, glnB
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 1 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P213
Unit cell:
a: 80.682Å b: 80.682Å c: 80.682Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.126 0.125 0.144
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

P(II) signal transduction proteins: nitrogen regulation and beyond.
Huergo et al. (2013)
5 more

5 mentions without citation

The RCSB Protein Data Bank: new resources for research and education.
Rose et al. (2013)
4 more