› 2xbt

X-ray diffraction
1.83Å resolution

Structure of a scaffoldin carbohydrate-binding module family 3b from the cellulosome of Bacteroides cellulosolvens: Structural diversity and implications for carbohydrate binding

Released:
DOI: 10.2210/pdb2xbt/pdb
PDB entry 2xbt coloured by chain, front view.
PDB entry 2xbt coloured by chain, side view.
PDB entry 2xbt coloured by chain, top view.
Source organism: Pseudobacteroides cellulosolvens
Primary publication:
Scaffoldin-borne family 3b carbohydrate-binding module from the cellulosome of Bacteroides cellulosolvens: structural diversity and significance of calcium for carbohydrate binding.
Yaniv O, Shimon LJ, Bayer EA, Lamed R, Frolow F
Acta Crystallogr D Biol Crystallogr 67 506-15 (2011)
PMID: 21636890

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190299 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulosomal scaffoldin Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 17.36 KDa
Source organism: Pseudobacteroides cellulosolvens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9FDJ9 (Residues: 958-1115; Coverage: 7%)
Gene name: scaA
Sequence domains: Cellulose binding domain
Structure domains: Endoglucanase-like

Ligands and Environments

1 bound ligand:
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I4122
Unit cell:
a: 83.193Å b: 83.193Å c: 96.139Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.191 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Continually emerging mechanistic complexity of the multi-enzyme cellulosome complex.
Smith et al. (2017)
8 other articles

3 mentions without citation

Structure of a family 3a carbohydrate-binding module from the cellulosomal scaffoldin CipA of Clostridium thermocellum with flanking linkers: implications for cellulosome structure.
Yaniv et al. (2013)
2 more