› 2xcb

X-ray diffraction
1.85Å resolution

Crystal structure of PcrH in complex with the chaperone binding region of PopD

Released:
DOI: 10.2210/pdb2xcb/pdb
PDB entry 2xcb coloured by chain, front view.
PDB entry 2xcb coloured by chain, side view.
PDB entry 2xcb coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis of chaperone recognition of type III secretion system minor translocator proteins.
Job V, Matteï PJ, Lemaire D, Attree I, Dessen A
J Biol Chem 285 23224-32 (2010)
PMID: 20385547

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129314 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulatory protein PcrH Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 15.49 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I325 (Residues: 21-160; Coverage: 84%)
Gene names: PA1707, pcrH
Structure domains: Tetratricopeptide repeat domain
PepD Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.2 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
UniProt:
  • Canonical: O50280 (Residues: 47-56; Coverage: 3%)
Gene name: pepD

Ligands and Environments

1 bound ligand:
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 44.37Å b: 97.72Å c: 73.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.262
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
11 more

6 mentions without citation

Molecular Targets and Strategies for Inhibition of the Bacterial Type III Secretion System (T3SS); Inhibitors Directly Binding to T3SS Components.
Hotinger et al. (2021)
5 more