› 2xcc

X-ray diffraction
2.13Å resolution

Crystal structure of PcrH from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb2xcc/pdb
PDB entry 2xcc coloured by chain, front view.
PDB entry 2xcc coloured by chain, side view.
PDB entry 2xcc coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis of chaperone recognition of type III secretion system minor translocator proteins.
Job V, Matteï PJ, Lemaire D, Attree I, Dessen A
J Biol Chem 285 23224-32 (2010)
PMID: 20385547

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein PcrH Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 15.49 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I325 (Residues: 21-160; Coverage: 84%)
Gene names: PA1707, pcrH
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 53.95Å b: 46.2Å c: 65.57Å
α: 90° β: 103.85° γ: 90°
R-values:
R R work R free
0.262 0.258 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
11 more

3 mentions without citation

Molecular Targets and Strategies for Inhibition of the Bacterial Type III Secretion System (T3SS); Inhibitors Directly Binding to T3SS Components.
Hotinger et al. (2021)
2 more