› 2xcj

X-ray diffraction
1.8Å resolution

Crystal structure of P2 C, the immunity repressor of temperate E. coli phage P2

Released:
DOI: 10.2210/pdb2xcj/pdb
PDB entry 2xcj coloured by chain, front view.
PDB entry 2xcj coloured by chain, side view.
PDB entry 2xcj coloured by chain, top view.
Source organism: Escherichia virus P2
Primary publication:
Crystal structure of the P2 C-repressor: a binder of non-palindromic direct DNA repeats.
Massad T, Skaar K, Nilsson H, Damberg P, Henriksson-Peltola P, Haggård-Ljungquist E, Högbom M, Stenmark P
Nucleic Acids Res 38 7778-90 (2010)
PMID: 20639540

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 11.03 KDa
Source organism: Escherichia virus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83VS7 (Residues: 1-99; Coverage: 100%)
Gene name: C
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand GOL 2 x GOL
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3221
Unit cell:
a: 91.103Å b: 91.103Å c: 80.754Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.149 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Bacteriophage protein-protein interactions.
Häuser et al. (2012)
1 more

4 mentions without citation

Crystal structure of the P2 C-repressor: a binder of non-palindromic direct DNA repeats.
Massad et al. (2010)
3 more