› 2xdc

X-ray diffraction
1.7Å resolution

Structure of linear gramicidin D obtained using Type I crystals grown in a lipid cubic phase.

Released:
DOI: 10.2210/pdb2xdc/pdb
PDB entry 2xdc coloured by chain, front view.
PDB entry 2xdc coloured by chain, side view.
PDB entry 2xdc coloured by chain, top view.
Source organism: Brevibacillus brevis
Primary publication:
Crystallizing transmembrane peptides in lipidic mesophases.
Höfer N, Aragão D, Caffrey M
Biophys J 99 L23-5 (2010)
PMID: 20682243

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GRAMICIDIN A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 16 amino acids
Theoretical weight: 1.88 KDa
Source organism: Brevibacillus brevis

Ligands and Environments

2 bound ligands:
Ligand 15P 4 x 15P
Ligand NA 1 x NA
3 modified residues:
Ligand FVA 6 x FVA
Ligand DLE 24 x DLE
Ligand DVA 12 x DVA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 30.5Å b: 62.56Å c: 30.53Å
α: 90° β: 99.97° γ: 90°
R-values:
R R work R free
0.181 0.179 0.214

Quick links

Citations

4 review citations

Lipidic cubic phase technologies for membrane protein structural studies.
Cherezov V. (2011)
3 more

5 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
4 more