› 2xe5

X-ray diffraction
2.28Å resolution

Molecular insights into clinically isolated OmpC mutants and their role in multi-drug resistance (OmpC26)

Released:
DOI: 10.2210/pdb2xe5/pdb
PDB entry 2xe5 coloured by chain, front view.
PDB entry 2xe5 coloured by chain, side view.
PDB entry 2xe5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Altered antibiotic transport in OmpC mutants isolated from a series of clinical strains of multi-drug resistant E. coli.
Lou H, Chen M, Black SS, Bushell SR, Ceccarelli M, Mach T, Beis K, Low AS, Bamford VA, Booth IR, Bayley H, Naismith JH
PLoS One 6 e25825 (2011)
PMID: 22053181

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin OmpC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 343 amino acids
Theoretical weight: 38.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K597 (Residues: 22-364; Coverage: 100%)
Gene names: BKL28_000773, D9D43_01315, DD762_02040, F7N46_15155, F9B07_08225, FKO60_02770, HL563_04725, ompC
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

9 bound ligands:
Ligand C8E 3 x C8E
Ligand HEX 10 x HEX
Ligand D12 6 x D12
Ligand OCT 10 x OCT
Ligand SO4 6 x SO4
Ligand D10 6 x D10
Ligand ASZ 1 x ASZ
Ligand OES 2 x OES
Ligand HP6 3 x HP6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P1
Unit cell:
a: 74.18Å b: 93.68Å c: 115.91Å
α: 98.74° β: 108.79° γ: 109.71°
R-values:
R R work R free
0.228 0.226 0.267
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance.
Fernández et al. (2012)
13 more

5 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
4 more